Do you want to assess anti-hypertensive effects of your molecule, extract or compound ?
Do you want to measure his effects on vascular contractility ?
We offer an extensive choice of in vitro, ex vivo and in vivo assays to give you answers.
In vivo exploration
- Arterial pressure analysis by Tail Cuff method
Conscious restrained rat or mice
The determination of systolic blood pressure and heart rate in conscious mice by tail-cuff method is a primary screen.
This non-invasive method allow the exploration on animals without an anaesthetic effect and to repeat experiments as many times as necessary in the protocol.
- Arterial pressure analysis by Telemetry
Conscious mice or rats
The telemetry allows the continuous recording of the systolic, diastolic, mean blood pressure and heart rate with implanted probe. The evaluation of several physiological signals without disturbing the animal can be realized during few days or few months.
It is possible to study the effects of treatment on long-term, remodeling following surgery, the development of a pathophysiology on transgenic animals…
Arterial hypertension (L-NAME, Angiotensin II) - Animal model: rat, mice
These models are characterized by the inhibition of endothelial NO synthase after L-NAME administration in drinking water or increase the plasma concentration of angiotensin II (AII) (stenosis of renal artery or implantation of osmotic minipompe of IIA). These models can be studied between 2-4 weeks.
- Pulmonary arterial pressure measurement (catheter-mediated)
Mean pulmonary arterial pressure is measured by the insertion of a catheter connected to a pressure transducer into the right jugular vein, then through the right atrium and the right ventricle into the pulmonary artery. Systemic arterial pressure is measured by another catheter inserted into the left femoral artery
Pulmonary arterial hypertension - Animal model: rat, mice
Model induced by hypoxia during 2-5 weeks (Hypoxic chamber).
Ex vivo exploration
(Vascular responses to different flow and pressure constraints)
In vitro exploration
Vascular and endothelial smooth muscle cells culture
(Ibidi® Pump System)
Ideal simulation of various physiological conditions – continuous unidirectional, oscillating, and pulsatile flow