Vascular

 

Do you want to assess anti-hypertensive effects of your molecule, extract or compound ?

Do you want to measure his effects on vascular contractility ?

We offer an extensive choice of in vitro, ex vivo and in vivo assays to give you answers.

 

 

 

 

Technology:

 

Tail Cuff Telemetry Isolated organs bath – Arteriograph – Mulvany Myograph Hypoxic chambers

 
 

 
 

 

In vivo exploration

 

• Arterial pressure analysis by Tail Cuff method

 

Conscious restrained rat or mice

The determination of systolic blood pressure and heart rate in conscious mice by tail-cuff method is a primary screen.

This non-invasive method allow the exploration on animals without an anaesthetic effect and to repeat experiments as many times as necessary in the protocol.

 

 

• Arterial pressure analysis by Telemetry

 

Conscious mice or rats

The telemetry allows the continuous recording of the systolic, diastolic, mean blood pressure and heart rate with implanted probe. The evaluation of several physiological signals without disturbing the animal can be realized during few days or few months.

It is possible to study the effects of treatment on long-term, remodeling following surgery, the development of a pathophysiology on transgenic animals…

 

 

Arterial hypertension (L-NAME, Angiotensin II) - Animal model: rat, mice

These models are characterized by the inhibition of endothelial NO synthase after L-NAME administration in drinking water or increase the plasma concentration of angiotensin II (AII) (stenosis of renal artery or implantation of osmotic minipompe of IIA). These models can be studied between 2-4 weeks.

 

 

 

• Pulmonary arterial pressure measurement (catheter-mediated)

 

Anaesthetized animal

Mean pulmonary arterial pressure is measured by the insertion of a catheter connected to a pressure transducer into the right jugular vein, then through the right atrium and the right ventricle into the pulmonary artery. Systemic arterial pressure is measured by another catheter inserted into the left femoral artery

 

Pulmonary arterial hypertension - Animal model: rat, mice

Model induced by hypoxia during 2-5 weeks (Hypoxic chamber).

 

 

 

 

Ex vivo exploration

 

Contractility of vascular rings – Mulvany Arteriograph  (Vascular responses to different flow and pressure constraints) Endothelial function (isolated organs bath) Histology, histomorphometry, immunohistology Biochemistry (protein expression and activation)

 

 

 

 

 

In vitro exploration

Adhesion, proliferation and migration (xCELLigence®) Vascular and endothelial smooth muscle cells culture   Endothelial cells under flow for simulation of blood vessels (Ibidi® Pump System) Ideal simulation of various physiological conditions – continuous unidirectional, oscillating, and pulsatile flow     Uniaxial stimulation of cell cultures (CellScale® MechanoCulture FX) Stretch protocol